The Elizabeth Glaser Pediatric AIDS Foundation (EGPAF) has worked in Lesotho since 2004, in collaboration with the Ministry of Health (MOH). EGPAF rapidly expanded access to comprehensive HIV/AIDS services throughout the country, establishing itself as a key HIV service implementation partner in Lesotho. Through this partnership and direct support to 121 facilities, EGPAF is well-placed to launch the UNITAID point-of-care early infant diagnosis (POC EID) project in Lesotho, which will significantly increase the number of HIV-positive infants receiving life-saving treatment in nine countries.